Variant Mining™ combines the top hits from a library to enable rapid discovery of highly active variants. This new sub-library can be assembled and delivered in pooled or arrayed format for your next round of evolution. You can continue to assay and combine hits as the feedstock for Variant Mining™ until you have found the highest level of fitness for your process relevant conditions. If you have not ordered the initial library from us, we are still happy to consult on whether it is appropriate for a variant mining workflow.

A typical workflow might look like:

Variants are “mined” and iterative libraries are built from pre-existing stocks of oligos/parts:

Variant Mining™ in action: replacement of the Saccharomyces cerevisiae adenosine biosynthesis pathway with the human pathway enabled by Variant Mining™ of PPAT sequences from humans, camels and whales to evolve a fully complementing PPAT enzyme with equivalent growth to Sc ADE4 trans complementation. DOI: 10.1093/nar/gkz1098


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